Skip to main content
. 2016 Aug 5;65(11):3505–3515. doi: 10.2337/db16-0634

Figure 3.

Figure 3

Direct PKA stimulation enhances α4 phosphorylation and adhesion. A: PKA-stimulated α4 phosphorylation. Untreated ECFCs or those treated with 8-Br-cAMP in the presence or absence of H89 were lysed, and levels of phospho-α4 (p-α4) and total α4 determined by Western blotting. Illustrated are representative blots. B: Illustrated are grouped data of phospho-α4–to–total α4 ratios normalized to untreated samples. n = 4. *P < 0.05. C: Cell adhesion. Cells variably incubated with 8-Br-cAMP as indicated were allowed to adhere to an S17 cell monolayer for 30 min. Adhesion was done in the presence of anti-α4 (PS/2), nonspecific (NS), or anti-VCAM antibodies, as indicated. The PKA dependence of enhanced adhesion was demonstrated by inhibition with H89. n ≥ 4. *P < 0.05.