Figure 5.

JH₂O₂ emitting potential in response to a 1-week HFD in 6N and 6J mice. Permeabilized fibers were prepared from WG (A and B) and RG muscle (C and D) of 6N and 6J mice fed a low-fat control diet or HFD for 1 week. JH₂O₂ emission was assessed in the presence of pyruvate (Pyr; 1 mmol) plus carnitine (Carn; 5 mmol) (A and C) and succinate (10 mmol) (B and D). #Difference between 6N and 6J mice within a respective diet group (P < 0.05). Isolate mitochondria were prepared from livers of 6N and 6J mice fed a low-fat control diet or HFD for 1 week. JH₂O₂ emission was assessed in the presence of succinate and succinate plus AF (E and F). E: Representative trace of an H₂O₂ emission experiment. F: Succinate and succinate/AF-supported JH₂O₂ emission. *Different from respective low-fat control group (P < 0.05), #difference between 6N and 6J mice (P < 0.05). Data are mean ± SEM; N = 6–8/group. Succ, succinate; wt, weight.