Figure 6.

Six-week HFD alters skeletal muscle mitochondrial JH₂O₂ emitting potential. Permeabilized fibers were prepared from WG (A, C, and E) and RG (B, D, and F) of 6N and 6J mice fed a low-fat control diet or HFD for 6 weeks. JH₂O₂ emission was assessed in the presence of pyruvate (Pyr; 1 mmol) and pyruvate (1 mmol) plus carnitine (Carn; 5 mmol) (A and B) and succinate (10 mmol) (C and D). JO₂ was assessed in permeabilized fibers prepared from WG (E) and RG (F) of 6N and 6J mice fed a low-fat control diet or HFD for 6 weeks. Substrate conditions were glutamate (10 mmol), malate (2 mmol), and succinate (10 mmol) in the absence (G/M/S) and presence (ADP) of ADP (2 mmol). *Different from respective low-fat control group (P < 0.05). #Difference between 6N and 6J mice within a respective diet group (P < 0.05). Data are mean ± SEM; N = 8–10/group. wt, weight.