Figure 1.

TAZ kd mice are protected from diet-induced obesity. A: Dox-inducible kd of TAZ protein in Tg (T) compared with NTg (N) mice was determined by immunoblot using isolated mitochondria (25 μg heart, 50 μg skeletal [Sk.] muscle, and 100 μg liver). Quantitation was performed with citrate synthase (Cit) as the loading control (n = 3–6). B: Body weights of Tg and NTg mice (n = 40). Body mass—both lean (C) and fat (D)—was measured (n = 17). E: Gonadal WAT stained with hematoxylin-eosin (HE; n = 5–10). F: Gonadal fat pad mass per body weight (b.w.) (n = 15–40). G: Hepatic macrovesicular (macrovesic.) steatosis was scored (n = 5–7). H and I: Plasma from fasted animals was separated into VLDLs, LDLs, and HDLs, and TGs and total cholesterol (Chol; unesterified and esterified) were measured (n = 3–4). rel. amt, relative amount. J: Livers from 10-month-old animals stained with HE or Oil Red O (ORO) (n = 5–14). Glucose tolerance (K) and insulin tolerance (L) tests (n = 12–28). Data are means ± SEMs. *P < 0.05 compared with NTg age-matched mice fed an identical diet; †P < 0.05, Tg + dox compared with all other groups; ‡P < 0.05, NTg + dox compared with all other groups.