Figure 5.

The assessment of mitochondrial function in TAZ kd mice. Quantification of H₂O₂ in heart (A), skeletal muscle (B), and liver (C) of 10-month-old Tg and NTg mice fed a diet containing dox. D–F: Hepatocytes were cultured in 10 mmol/L glucose, and basal respiratory (D), ATP-sensitive (oligomycin-sensitive; E), ATP-insensitive (i.e., heat; F), and maximum (max; FCCP; G) OCRs were measured; values are expressed per milligram of cellular protein (n = 4–5). H–K: Hepatocytes were cultured in 0.175 mmol/L palmitate-BSA in the presence of 40 μmol/L etomoxir or vehicle (water), and basal respiratory (H), ATP-sensitive (I), ATP-insensitive (J), and maximum (K) oxygen consumption were measured (n = 3–4). Values are means ± SEMs. *P < 0.05 compared with NTg age-matched mice fed an identical diet.