Fig 1. Using an siRNA library to identify genes involved in Andes virus infection of primary human lung cells.

Andes virus (ANDV)-infected primary human macrovascular endothelial lung cells (HMVEC-L) after siRNA administration (blue) were compared with a negative control set and are presented as the percentage of ANDV-infected cells treated with non-targeting siRNA (NT). The results are shown as averages (± SD) of 4 independent sets of experiments. Negative control set includes non-targeting siRNA, RISC-free siRNA, and siRNAs targeting GAPDH, cyclophilin B, and lamin A/C. siRNA pools against the ANDV N segment were used to demonstrate positive knockdown. ANDV infection (shown in red) is significantly decreased after siRNA knockdown (p < 0.05).