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. 2016 Oct 25;11(10):e0164768. doi: 10.1371/journal.pone.0164768

Fig 5. siRNA profiling of selected genes required for recombinant ANDV pseudovirion infection.

Fig 5

Gene-specific siRNA against integrin β3 (ITB3), DNM2, CLTC, AP2M1, CDC42, or ARF6, or the non-targeting siRNA transfection control (NT), were transfected into HMVEC-L at the concentration of 100 nM for 48 h. Cells were infected with pseudovirions (MOI = 1) for 48 h. All pseudovirions consisted of an HIV backbone with firefly luciferase, and were pseudotyped with ANDV GPC (ANDV) or vesicular stomatitis virus G protein (VSV). Effects of gene knockdown were measured by quantifying luciferase activity in the host genome. Solid bars represent ANDV, and open bars represent VSV. Luciferase activity is expressed as units relative to non-targeting siRNA control (% infection). The effect of ITB3-specific siRNA knockdown is shown in the inset. Results presented are the averages (± SD) of 10 experiments (n = 10), and significant decreases in pseudovirion infection (p < 0.05) after siRNA knockdown are shown with an asterisk (*).