Table 2. Chemical inhibitors of endocytic pathways used in this study.

Inhibitor	Endocytosis Pathway Blocked	Specific Activity	Toxicity to HMVEC-L (CC20)
Dynasore	Clathrin and caveolin	Blocks dynamins	87 μM
Chlorpromazine(CPZ)	Clathrin	Prevents coated pit formation by blocking AP2 binding to receptor	22 μM
Pitstop 2	Clathrin	Prevents coated pit formation by blocking attachment to clathrin heavy chain	16 μM
Methyl-β-cyclodextrin (MβCD)	Caveolin, clathrin, and macropinocytosis	Extracts cholesterol from cells	2 mM
5-(N-ethyl-N-isopropyl) amiloride (EIPA)	Macropinocytosis	Inhibits Na+/H+ exchange	51 μM
LY294002	Macropinocytosis	Inhibits PI3K α/β/δ	13 μM
Cytochalasin D	Clathrin and macropinocytosis	Disrupts actin polymerization	300 nM