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. 2016 Jul 27;12(10):1738–1758. doi: 10.1080/15548627.2016.1196318

Figure 2.

Figure 2.

Alteration of autophagic activity affects viability of CSFV-infected cells. (A and C) PK-15 (A) and 3D4/2 (C) cells were pretreated with rapamycin (100 nM) or DMSO for 1 h. Cells were then mock infected or infected with CSFV at an MOI of 1. After 1 h of virus absorption at 37°C, the cells were incubated in fresh medium containing rapamycin (100 nM ) or DMSO for 0, 12, 24 and 48 h. Cells only treated with rapamycin were considered as one control. Meanwhile, 2 cell lines were transfected with shRNAs targeting BECN 1 or LC3B or scramble shRNA for 48 h, followed by mock infection and CSFV infection for 0, 12, 24 and 48 h at the same MOI. Then the cell viability was determined by the CCK-8 assay. The data represent the mean ± SD of 3 independent experiments. One-way ANOVA test; test of homogeneity of variances, P > 0.05, LSD (L) was used for correction of post-hoc test. (B and D) PK-15 (B) and 3D4/2 (D) cells were pretreated and transfected as described in (A and C), followed by mock infection and CSFV infection for 48 h at an MOI of 1. Percentage of cell death was then detected using a live-dead cell staining solution and analyzed by flow cytometry. The data represent the mean ± SD of 3 independent experiments. One-way ANOVA test; test of homogeneity of variances, P > 0.05, LSD (L) was used for correction of post-hoc test. **, P < 0.01; ***, P < 0.001; #, P > 0.05.