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. 2016 Jul 18;12(10):1902–1916. doi: 10.1080/15548627.2016.1208889

Figure 5.

Figure 5.

NFE2L2 deficiency leads to accumulation of aggregated HsMAPT. (A) Immunohistochemical analysis of HsMAPT expression in hippocampus of 13-mo-old AT-Nfe2l2-WT and AT-nfe2l2-KO mice stained with anti HT7 or AT8 antibodies that recognize total (HT7) or phosphorylated (AT8) HsMAPT. (B) Hippocampal tissue lysates from AT-Nfe2l2-WT and AT-nfe2l2-KO mice were separated into sarkosyl-soluble (SS) and sarkosyl-insoluble (SI) fractions. Total HsMAPT and MmMAPT levels were determined using anti-TAU46 antibody that recognizes MAPT from both species. (C) Densitometric quantification of representative blots from (B). (D) Hippocampal tissue lysates from AT-Nfe2l2-WT and AT-nfe2l2-KO mice were separated into sarkosyl-soluble (SS) and sarkosyl-insoluble (SI) fractions and analyzed with anti-PHF1 antibody that recognizes human p-HsMAPT. (E) Densitometric quantification of representative blots from (D). For (C) and (E), data are mean ± SEM (n = 6). Statistical analysis was performed with the Student t test. *, p < 0.05; comparing AT-Nfe2l2-WT and AT-nfe2l2-KO groups.