Figure 5.

The involvement of newly synthesized proteins in starvation-induced autophagy. (A) HeLa cells were transiently transfected with a nonspecific siRNA or the ATP5B-, RACK1-, HSPE1-, PNP- or SLC25A3- targeted siRNA and treated for 2 h starvation alone or in combination with BAF (25 nM). Total RNA was isolated from HeLa cells and the mRNA levels of the above genes were quantified by real-time PCR. GAPDH was used as the internal control. (B) HeLa cells as described in (A) were subjected to immunoblotting of LC3 and SQSTM1 using cell lysates. ACTB served as the loading control.