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. Author manuscript; available in PMC: 2017 Feb 22.
Published in final edited form as: Lab Invest. 2016 Aug 22;96(11):1198–1210. doi: 10.1038/labinvest.2016.89

Figure 3.

Figure 3

Isolation of hepatic mast cells from BDL post 14 days. By enzymatic digestion and magnetic bead binding a virtually pure population of mast cells was obtained from BDL rat liver day 14. Toluidine blue staining indicates a population of mature mast cells as shown by intact granules (black arrows, beads are indicated by red arrows). Image is 20× magnification. The purity of isolated mast cells (~95%) was determined by counting toluidine positive blue mast cells prior to bead attachment and after. The total number of isolated mast cells totaled 2.12 million/ml and the average size was 8.2 μM.