Figure 3.
Type I IFNs reverse the immunosuppression of MSCs. (a) MSCs were cocultured with fresh splenocytes plus anti-CD3 and anti-CD28 with or without l-NMMA (1 mm) or IFNα (2500 U/ml) for 48 h. Cell proliferation was evaluated by 3H-Tdr incorporation assay. (b, c) MSCs were plated at different concentrations and coculture with fresh splenocytes (1 × 106/well) plus anti-CD3 and anti-CD28. Cell proliferation was assayed after 48 h. Proliferation values are means±s.d. of four wells from a representative of two independent experiments. **P<0.01.