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. Author manuscript; available in PMC: 2016 Nov 14.
Published in final edited form as: Oncogene. 2016 Apr 25;35(45):5850–5859. doi: 10.1038/onc.2016.123

Figure 1. The entire cellular pool of BCAR3 is in complex with Cas.

Figure 1

BT549 and MDA-MB-231 cell lysates were subjected to three serial Cas (A) or BCAR3 (B) immunoprecipitations (IP). Pre-IP lysates were separated on 8% SDS-PAGE (lanes 1 and 8) together with the proteins present in the IPs (lanes 5–7, 12–14) and post-IP lysates (2–4, 9–11). Proteins were immunoblotted with antibodies recognizing the designated proteins. The pre-IP lysate is 10% of the amount of protein used for the initial IP.