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. Author manuscript; available in PMC: 2016 Nov 14.
Published in final edited form as: Oncogene. 2016 Apr 25;35(45):5850–5859. doi: 10.1038/onc.2016.123

Figure 5. BCAR3/Cas interactions are required for BCAR3 dependent Rac activity.

Figure 5

BT549 cells were transfected with plasmids encoding GFP, GFP-WT BCAR3, or GFP-L744E/R748E BCAR3 and incubated for 24 hours. Cells were held in suspension for 90 minutes, then plated on 10μg/ml fibronectin for 1 hour. (A) GTP-bound Rac1 was isolated from whole cell lysates by incubation with PAK-1-binding domain agarose. Bound proteins (middle panel) and total Rac1 (bottom panel) were detected by immunoblotting with a Rac1 antibody, and BCAR3 expression was confirmed with a BCAR3-specific antibody (top panel). (B) Quantification of the relative GTP-Rac1 level is shown. Data presented are the mean ± SEM of 3 independent experiments.