Table 1.

Physiological features of the sequenced Oxalobacteraceae strains and related species^*.

Feature	Janthinobacterium									Duganella
	HH100	HH102	HH103	HH104	HH106	HH107	5059	1522	9628	HH101	HH105	23865	16928	HH01
Violacein synthesis (μg/ml)	1.27 ± 0.12	1.54 ± 0.4	1.19 ± 0.02	1.83 ± 0.08	0.15 ± 0.07	0.93 ± 0.26	0.37 ± 0.02	0.03 ± 0.01	−	6.33 ± 0.15	3.36 ± 0.76	−	−	1.98 ± 0.02
Amylolytic activity	−	−	−	−	−	−	−	−	+	+	+	+	+	+
Lipolytic activity	+	+	+	+	+	+	+	++	+	+++	+++	+++	+	+++
Proteolytic activity	++	++	++	++	++	++	++	−	+	(+)	(+)	−	−	(+)
Chitinoclastic activity	++	++	++	++	++	++	++	++	++	+	+	−	−	+

^*Strains sequenced in this work are indicated by gray shadings. 9628 refers to J. agaricidamnosum, 1522 to J. lividum, 23865 to D. phyllosphaerae and 16928 to D. zoogloeoides. Violacein (μg/ml) was extracted after growth for 24 h in R2A. Amylolytic and proteolytic activities were measured after 72 h of growth on plates and lipolytic activities after 144 h of growth on solid media containing starch, skim milk, or tributyrin. Activities were recorded as diameter of clearing zones around individual colonies. Chitinolytic activity was determined after 1 week of growth in M9 lacking glucose and supplemented with chitin. +, enzymatic was observed; ++, strong activity; +++, very strong activity; (+), weak activity; –, no activity observed, data are mean values of at least three independent biological test; ± indicates the standard deviations.