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. 2016 Aug 17;92(4):425–443. doi: 10.1007/s11103-016-0522-3

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — Anacardic acid inhibits UV-B induced H3K9,14diac enrichment and expression of specific UVR8-regulated genes. A Relative UV-B induced enrichment of H3K9,14diac at selected loci, assayed by ChIP-qPCR, and B fold UV-B induction of transcripts of selected genes, measured by RT-qPCR, in plants treated with increasing concentrations of anacardic acid (AA). Plants were infiltrated with the inhibitor for 15 min and then exposed (or not in controls) to 1.5 μmol m⁻² s⁻¹ narrowband UV-B for 1 h. Plants were harvested immediately for ChIP assays or harvested after 2 h in darkness for RT-qPCR. Error bars represent SD (n = 3)