Figure 4.

Accumulation of acetyl-CoA (A) and fatty acids (B) during growth in standard BG11 with ammonia as nitrogen source in wild type (white bars), the P_II mutant (gray bars), the P_II complemented strain (checked bars) and the P_II-S49E complemented strain (gray dotted bars). Values represent the mean of three biological replicates. Differences in acetyl-CoA levels are statistically significant throughout growth between wild type and the P_II mutant (p < 0.05; unpaired t-test). Differences in fatty acid levels between wild type and the P_II mutant are statistically significant within the first 48 h of growth (p < 0.05; unpaired t-test).