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. 2016 Oct 26;7:394. doi: 10.3389/fphar.2016.00394

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Copyright © 2016 Bi, Zhang, Guo, Bi, Liu, Zou and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes of claudin-5 expression in NC, CO and NBP+CO groups. A large number of claudin-5-positive vessels were observed in NC subjects, and the positive levels of claudin-5 protein were notably reduced on day 1, followed by gradually restoration to approximate normal level on day 14 in CO group. The levels of claudin-5-positive vessel in NBP+CO group were higher than that in CO group (n = 4, P < 0.05). (A) The expressions of claudin-5 positive cells in each group; (B) The OD value alterations of claudin-5 positive cells in different groups (n = 4). ^∗Compared with NC group, P < 0.05; ^#compared with CO group at the same time point, P < 0.05 (F = 18.251∼32.817). Scale bar = 30 μm.