FIG 2 .

Design and fluorescence analysis of Φ²DRM constructs. (a) Schematics of the various Φ²DRMs used in the study. The expression of mVenus in Φ²GFP10 and all Φ²DRMs is driven from the constitutive P_L(L5) promoter. A unique promoter, listed on the right of each schematic, drives the expression of tdTomato in each Φ²DRM. Transcription terminators, placed upstream from tdTomato and downstream from mVenus to avoid transcriptional read-through, are indicated by black boxes. Arrows indicate the relative positions of promoters. (b) Expression kinetics of mVenus from P_L(L5) in M. tuberculosis by time lapse microscopy. Expression of mVenus was detectable after approximately 4 h of phage infection. (c) Quantitative plot of the kinetics of mVenus expression over time. Fluorescence intensity increased progressively over time, plateauing approximately 18 h after the addition of phage. AU, arbitrary units. The error bars represent SD of fluorescent intensity measured at three independent fields.