FIG 2 .
Passive immunization with anti-EbpAFull and anti-EbpANTD antibodies prevents E. faecalis CAUTI. Mice (n = 10) were given a dose of 100 µl of PBS sera or 100 µl of anti-EbpAFull or anti-EbpANTD with a titer of 1 × 107. (A) Experimental timeline. Sac, time of mouse sacrifice. (B and C) Detection of EbpAFull and EbpANTD antibodies in bladder (B) and in urine (C) analyzed by diluting each sample 1:100 before serial dilution. (D and E) Prophylaxis treatment performed using 1 dose at 4 h prior infection (D) or 2 doses at 4 h prior infection and 12 h postinfection (hpi) (E). Values represent means ± SEM. The Mann-Whitney U test was used for mouse experiments; P < 0.05 was considered statistically significant. *, P < 0.05; **, P < 0.005; ***, P < 0.0005; ****, P < 0.00005; ns, values were not statistically significantly different. The horizontal bar represents the median value. The horizontal broken line represents the limit of detection (LOD) of viable bacteria. Animals that lost the catheter were not included in this work.