FIG 8 .

(A) Duplication times of P. aeruginosa strains. Duplication times (in minutes) of exponentially growing cells of strain PAO1 and strains derived from strain PAO1 in LB broth are shown. Values are means ± SD from three independent experiments performed for each strain. The values that are significantly different (P < 0.0001 by Student’s t test) from the value for the wild-type strain are indicated by a bar and asterisk. (B) Galleria mellonella killing assays with PAO1-derived strains. G. mellonella larvae were infected as described in Materials and Methods. LD₅₀s were determined as previously described (53). To analyze the differences between the obtained LD₅₀s, Student’s t test was performed with the values of the three independently obtained LD₅₀s. The values that are significantly different (P < 0.005 by Student’s t test) from the value for the wild-type strain are indicated by a bar and asterisk.