FIG 1 .

Gly-MCA alters the gut microbiota population and composition. HFD-fed mice were treated for 5 weeks with Gly-MCA (10 mg/kg). (A) Generalized Unifrac analysis of the total population of the gut microbiome of cecal contents from vehicle- and Gly-MCA-treated mice and mice treated with Gly-MCA plus GW4064. (B) 16S rRNA gene sequencing analysis at the phylum level of the cecal contents and ratio of Firmicutes to Bacteroidetes. (C to E) 16S rRNA gene sequencing analysis at the class (C), family (D) and genus (E) levels in the cecal contents. Data are presented as means ± SD (n = 5 per group). *, P < 0.05; **, P < 0.01 (compared with vehicle treatment). #, P < 0.05; ##, P < 0.01 (compared with Gly-MCA treatment of HFD-fed mice). Data were analyzed with a one-way ANOVA with Tukey’s correction.