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. Author manuscript; available in PMC: 2016 Oct 26.
Published in final edited form as: Prog Biophys Mol Biol. 2015 May 1;118(3):119–129. doi: 10.1016/j.pbiomolbio.2015.04.005

Fig. 10.

Fig. 10

Over-expression of EnvZc and EnvZc-mEos inhibits wildtype EnvZ activity. (A) β-galactosidase activity of ompF-lacZ stimulated by EnvZc (shaded bars) and EnvZc-mEos (striped bars). Cells were grown in low osmolality A media for 4 h (157 mOsm/Kg). Measurements were performed in triplicate and the mean and standard deviation was determined. Data were plotted as a percentage of the wildtype activity, where wildtype is 100%. Below the graph is a western blot. EnvZ wildtype protein was detectible (left lane), but was absent in the envZ null strain (blot lane 2) and was visible only at 0.2% and 0.002% arabinose (blot lanes 3 and 4, respectively). At lower arabinose concentrations, EnvZc protein was not detected, but the EnvZc activity was similar to wildtype (82–93%). The EnvZc-mEos activity was similar to wildtype (83–85%). (B) β-galactosidase activity of ompC-lacZ stimulated by EnvZc (shaded bars) and EnvZc-mEos (striped bars). Cells were grown in A media +15% sucrose (736 mOsm/Kg) for 4 h and measurements were performed as described in (A). As was observed with ompF-lacZ, EnvZc-mEos was essentially identical to wildtype in supporting OmpR-dependent transcription (84–104%).