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. 2016 Oct 20;7:13173. doi: 10.1038/ncomms13173

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Copyright © 2016, The Author(s)

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Following non-automated transduction of CD34⁺ cell products from HPC-A donors 1 and 2 in the absence of UM729, aliquots of the final cell product were cultured in liquid media consisting of recombinant human growth factors G-CSF, SCF, TPO, Flt3-L, IL-3 and IL-6 for subsequent real-time PCR to determine VCN (a), or in methylcellulose media containing the same recombinant human growth factors for colony-forming assay (b).