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. 2016 Oct 20;7:13173. doi: 10.1038/ncomms13173

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Two animals (Z13105 and Z13083) received autologous, LV gene-modified CD34⁺ cells produced under semi-automated conditions following myeloablative TBI. (a) Graphs depict haematopoietic recovery by ANCs (open black circles○) and platelet counts (closed black circles) on the primary y axis, engraftment of gene-modified PB granulocytes (closed green squares) and lymphocytes (open green squares) and measured tacrolimus levels in serum (closed orange circles) on the secondary y axis as a function of time after transplantation (x axis). (b) Percent of gene-modified (eGFP⁺) lymphocytes expressing CD3, and the percentage of eGFP⁺/CD3⁺ cells expressing CD4 and/or CD8 observed in PB (y axis) over time after transplant (x axis). (c) Highly polyclonal engraftment of LV gene-modified NHP CD34⁺ haematopoietic cells following point-of-care production and transplant. Bar graphs represent the clonal diversity of LV gene-modified PB leukocytes collected at day +40 after transplant into autologous recipients as determined by genomic locus of integration. Clonal integration site sequences constituting ≥1% of all sequences captured are indicated by boxes in ascending order of frequency. Coloured boxes indicate clones identified across time points. Total number of clones identified is listed atop each bar.