Figure 3. HSP60 levels determine the proliferative capacity of the crypt.

(a) IF co-staining of HSP60 and Ki67 on jejunal sections including detailed images in higher magnification of Hsp60^flox/flox controls and Hsp60^Δ/ΔIEC mice (DAPI stains nuclei in cyan; dotted lines indicate localization of cryptal epithelium). Quantification of Ki67-positive cells in HSP60-positive and HSP60-negative crypts (N=5; 2 regions per mouse). (b) Parallel evaluation as in (a) for Chop^−/− Hsp60^Δ/ΔIEC mice and Chop^−/− Hsp60^flox/flox controls. Lines in the dot plots indicate mean numbers. (a,b,c), significantly different from each other. One-way analysis of variance (ANOVA) and appropriate post-hoc test were used for statistical analysis.