Fig. 7.

SPR sensorgrams showing mTlD does not bind directly to Tsg.

Immobilised proteins were cross-linked to a CM5 chip via amine coupling. 830 RU of Tsg was immobilised on the chip and two analytes were injected onto the immobilised Tsg: 500 nM mTlD (black response curve) and 500 nM mTlDC4C5 (red response curve). (B) Two analytes were injected onto the immobilised mTlD (2400 RU): 500 nM Tsg (black response curve) and 500 nM ΔN-chordin (red response curve). (C) ∆N-chordin cleavage assay by mTld in the presence of increasing amounts of Tsg (0:1, 0.1:1 0.25:1, 0.5:1, 1:1 and 4:1 molar ratio of Tsg:∆N). The emergence of the chordin cleavage product, intermediate fragment (IF) is labelled. A plot of the percentage of cleaved chordin against Tsg:chordin ratio is shown. (D) Schematic diagram demonstrating proposed mechanism of conformational change in chordin following Tsg binding exposing tolloid recognition sites.