Table 1.

Measured and calculated gating charges of class A GPCRs.

Receptor	Gating charge (e)	Reporter method	Refs
m1 muscarinic:
wt	0.72, 0.76a	FRET	[18••]
m2 muscarinic:
wt	0.55	Electrophysiology	[11]
wt	0.53 (Na+)	MD simulation	[10••]
wt	0.52 (proton)	MD simulation	[10••]
wt	0.7, 0.85	Electrophysiology	[32]
D692.50A	NR	Electrophysiology	[11]
W993.28A	0.8	Electrophysiology	[11]
D1033.32A	0.5	Electrophysiology	[11]
Y1043.33A	0.54	Electrophysiology	[11]
S1073.36A	0.49	Electrophysiology	[11]
D1203.49N	0.66	Electrophysiology	[32]
D1203.49N-R1203.50N	0.52	Electrophysiology	[11]
D1203.49N-R1203.50N	NR	Electrophysiology	[32]
Y4036.51A	0.57	Electrophysiology	[11]
α2A-adrenergic
wt	0.5	FRET	[19]
δ-opioid:
wt	0.42 (Na+)	MD simulation	[10••]
N1313.35V	0.63 (Na+)	MD simulation	[10••]

NR, not resolved.

^aPrecise value depends on methodology used.