Figure 1.

Characterization of AF-MSCs. (a) Representative image of spindle-shaped AF-MSCs. Scale bar = 400 μm. (b) Expression of cell surface markers CD45, CD34, CD105, and CD90 measured by using FACS and FITC or PE labeled antibodies. The data was represented as mean with standard deviation (n = 3). (c) Expression of stem cells pluripotency markers Oct4, Sox2, Nanog, and Rex1 as determined by RT-qPCR. The data were normalized to GAPDH and presented as mean ± SD (n = 3). (d) AF-MSCs after induction of osteogenic differentiation for 15 days, stained with Alizarin Red. Calcified extracellular matrix deposition is colored red proving osteogenic differentiation.