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. 2016 Oct 12;2016:6465307. doi: 10.1155/2016/6465307

Figure 3.

Figure 3

MicroRNA expression analysis during osteogenic differentiation of AF-MSCs. (a) Relative expression of miR-21 and miR-17 that are related to pluripotency maintenance in stem cells. (b) Relative expression of microRNAs 34a, 223, and 148b, which are upregulated or downregulated during osteogenic differentiation. MicroRNA expression was determined by RT-qPCR and data, normalized to RNU48, are presented as n-fold change over control. Results are shown as mean ± SD (n = 3); ∗ P ≤ 0.05, ∗∗ P ≤ 0.001, and ∗∗∗ P ≤ 0.0001 indicate significant differences from control.