Figure 5.

Immunostaining of CPCs shows expression and localization of the LTCC and IP₃R. CPCs were fixed, permeabilized, and incubated with primary antibodies against the L-type Ca²⁺ channel (LTCC, Cav2.1 subunit), type 2 inositol 1,4,5-trisphosphate receptor (IP₃R), or type 2 ryanodine receptor (RyR). Cells were then incubated with either Alexa Fluor 488 (IP₃R and RyR; green) or Alexa Fluor 647 (LTCC; red). Nuclei are stained with DAPI (blue). Expression of the IP₃R and LTCC is shown in (a), while expression of the LTCC and the RyR is shown in (b). Cells were also incubated with only secondary antibodies to determine nonspecific binding in (c). Images are representative of 3 replicates.