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. 2016 Oct 26;7:1539. doi: 10.3389/fpls.2016.01539

FIGURE 2.

FIGURE 2

Differential real-time quantification of gene expression validates our transcriptome approach. Demonstrated here is down-regulation of homologs of meiotic genes ASY1 (A–C) and MPS1 (D–F), accompanied by upregulation of stress-response genes, a GATA type (ZML1) transcription factor (G–I) and JASMONATE-ZIM-DOMAIN PROTEIN 9 (JAZ9) (J,K). Sex-1 versus Apo-1 seedlings-derived RNA-seq differential expression data (first column) were validated by real-time qRT-PCR (second column), and the expression analyses were further assayed by qRT-PCR in corresponding floral buds of the pre-meiotic MMC-stage (Supplementary Figure S3) (third column). Apo-2 was used as an additional apomictic genotype for qRT-PCR comparisons. FPKM – fragments per kilobase per million. Statistical significance was calculated using t-test; significance levels: ∗∗α ≤ 0.01; ∗α ≤ 0.05; NS, not significant. t-test results for comparisons between groups for reproductive mode and ploidy (Sex vs. Apo, and diploid vs. triploid) are indicated below the respective bar chart.