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. 2016 Oct 26;6:36008. doi: 10.1038/srep36008

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Copyright © 2016, The Author(s)

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(A) Relative expression of related imprinted genes (GRB10, IGF2, PEG1, PEG10, H19 and NNAT) in fresh and aged oocytes in vitro aging with or without cumulus cells for 24 h or 48 h. (B) The development of blastocysts from fresh and aged oocytes in vitro aging for 24 h after parthenogenetic activation. (C) Relative expression of related imprinted genes (GRB10, IGF2, PEG1, PEG10, H19 and NNAT) in blastocysts from fresh and aged oocytes in vitro aging for 24 h after parthenogenetic activation. All graphs show mean ± s.e.m. Abbreviations used in this and all subsequent figures: COC, cumulus oocyte complex; DO, denuded oocyte; IVA, in vitro aging. a–c: Values without a common letter in their superscripts differ significantly (P < 0.05).