| pGK416 |
Expression vector containing PGK1 promoter, CEN/ARS single-copy origin and URA3 marker |
33 |
| pGK416-EGFP |
EGFP expression, in pGK416 |
33 |
| pGK416-AcGFP1 |
AcGFP1 expression, in pGK416 |
This study |
| pGK416-TagGFP2 |
TagGFP2 expression, in pGK416 |
This study |
| pGK416-mUkG1 |
mUkG1 expression, in pGK416 |
This study |
| pGK416-ZsGreen |
ZsGreen expression, in pGK416 |
34 |
| pGK416-yEGFP |
yEGFP (codon-optimized EGFP) expression, in pGK416 |
This study |
| pGK416-yAcGFP1 |
yAcGFP1 (codon-optimized AcGFP1) expression, in pGK416 |
This study |
| pGK416-yTagGFP2 |
yTagGFP2 (codon-optimized TagGFP2) expression, in pGK416 |
This study |
| pGK416-ymUkG1 |
ymUkG1 (codon-optimized mUkG1) expression, in pGK416 |
This study |
| pGK416-yZsGreen |
yZsGreen (codon-optimized ZsGreen) expression, in pGK416 |
This study |
| pGK416-ymWasabi |
ymWasabi (codon-optimized mWasabi) expression, in pGK416 |
This study |
| pGK416-ymNeonGreen |
ymNeonGreen (codon-optimized mNeonGreen) expression, in pGK416 |
This study |
| pGK416-EGFP-F |
EGFP-FLAG expression, in pGK416 |
This study |
| pGK416-AcGFP1-F |
AcGFP1-FLAG expression, in pGK416 |
This study |
| pGK416-TagGFP2-F |
TagGFP2-FLAG expression, in pGK416 |
This study |
| pGK416-mUkG1-F |
mUkG1-FLAG expression, in pGK416 |
This study |
| pGK416-ZsGreen-F |
ZsGreen-FLAG expression, in pGK416 |
This study |
| pGK416-yEGFP-F |
yEGFP (codon-optimized EGFP) -FLAG expression, in pGK416 |
This study |
| pGK416-yAcGFP1-F |
yAcGFP1 (codon-optimized AcGFP1) -FLAG expression, in pGK416 |
This study |
| pGK416-yTagGFP2-F |
yTagGFP2 (codon-optimized TagGFP2) -FLAG expression, in pGK416 |
This study |
| pGK416-ymUkG1-F |
ymUkG1 (codon-optimized mUkG1) -FLAG expression, in pGK416 |
This study |
| pGK416-yZsGreen-F |
yZsGreen (codon-optimized ZsGreen)-FLAG expression, in pGK416 |
This study |
| pGK416-ymWasabi-F |
ymWasabi (codon-optimized mWasabi)-FLAG expression, in pGK416 |
This study |
| pGK416-ymNeonGreen-F |
ymNeonGreen (codon-optimized mNeonGreen)-FLAG expression, in pGK416 |
This study |
| pBlueScript II KS(+) |
Cloning vector |
Agilent Technologies |
| pBlue-FIG1pt-ZsGreen |
PFIG1
(300 bp)-URA3-ZsGreen-TFIG1
(200 bp) in pBlueScript II KS(+) |
34 |
| pBlue-FIG1t |
TFIG1
(200 bp) in pBlueScript II KS(+) |
This study |
| pBlue-UFt-AcGFP1 |
C-terminus of FIG1 (50 bp)-AcGFP1-URA3-TFIG1
(200 bp) in pBlueScript II KS(+) |
This study |
| pBlue-UFt-TagGFP2 |
C-terminus of FIG1 (50 bp)-TagGFP2-URA3-TFIG1
(200 bp) in pBlueScript II KS(+) |
This study |
| pBlue-UFt-mUkG1 |
C-terminus of FIG1 (50 bp)-mUkG1-URA3-TFIG1
(200 bp) in pBlueScript II KS(+) |
This study |
| pBlue-UFt-ZsGreen |
C-terminus of FIG1 (50 bp)-ZsGreen-URA3-TFIG1
(200 bp) in pBlueScript II KS(+) |
This study |
| pBlue-UFt-yEGFP |
C-terminus of FIG1 (50 bp)-yEGFP-URA3-TFIG1
(200 bp) in pBlueScript II KS(+) |
This study |
| pBlue-UFt-yAcGFP1 |
C-terminus of FIG1 (50 bp)-yAcGFP1-URA3-TFIG1
(200 bp) in pBlueScript II KS(+) |
This study |
| pBlue-UFt-yTagGFP2 |
C-terminus of FIG1 (50 bp)-yTagGFP2-URA3-TFIG1
(200 bp) in pBlueScript II KS(+) |
This study |
| pBlue-UFt-ymUkG1 |
C-terminus of FIG1 (50 bp)-ymUkG1-URA3-TFIG1
(200 bp) in pBlueScript II KS(+) |
This study |
| pBlue-UFt-yZsGreen |
C-terminus of FIG1 (50 bp)-yZsGreen-URA3-TFIG1
(200 bp) in pBlueScript II KS(+) |
This study |
| pGK426 |
Expression vector containing PGK1 promoter, 2 μ origin and URA3 marker |
33 |
| pGK426-GPTK |
URA3-PSTE18-kanMX4-TSTE18 in pGK426 |
42 |
| pUSTE18p-Gγcyto-HIS3t |
URA3-PSTE18-Gγcyto-TPGK1-THIS3 in pGK426 |
45 |
| pUMGP-GγMFcH |
URA3-PSTE18-Gγcyto-Fc-TPGK1 in pUSTE18p-Gγcyto-HIS3t |
42 |
| pGK413 |
Expression vector containing PGK1 promoter, CEN/ARS single-copy origin and HIS3 marker |
33 |
| pGK413-ZWTmem |
ZWT and C-terminus of Ste18 (9 a.a.) fusion expression, in pGK413 |
40 |
| pGK413-ZK35Amem |
ZK35A and C-terminus of Ste18 (9 a.a.) fusion expression, in pGK413 |
40 |
| pGK413-ZI31Amem |
ZI31A and C-terminus of Ste18 (9 a.a.) fusion expression, in pGK413 |
40 |
| pGK413-Z955mem |
Z955 and C-terminus of Ste18 (9 a.a.) fusion expression, in pGK413 |
40 |
| pGK426-GPTK |
URA3-PSTE18-kanMX4-TSTE18 in pGK426 |
42 |
| pUMGPTK-Gpa1N-Fc |
URA3-PSTE18-PPGK1-Gpa1N (9 a.a.)-Fc-TPGK1-kanMX4-TSTE18 in pGK426-GPTK |
45 |
| pUMGPTK-Fc-Ste18C |
URA3-PSTE18-PPGK1-Fc-Ste18C (9 a.a.)-TPGK1-kanMX4-TSTE18 in pGK426-GPTK |
45 |
| pUSTE18p-Gγcyto-HIS3t |
URA3-PSTE18-Gγcyto-TPGK1-THIS3 in pGK426 |
45 |
| pUSTE18p-Gγcyto-ZWT-H |
URA3-PSTE18-Gγcyto-ZWT-TPGK1 in pUSTE18p-Gγcyto-HIS3t |
45 |
| pUSTE18p-Gγcyto-ZK35A-H |
URA3-PSTE18-Gγcyto-ZK35A-TPGK1 in pUSTE18p-Gγcyto-HIS3t |
45 |
| pUSTE18p-Gγcyto-ZI31A-H |
URA3-PSTE18-Gγcyto-ZI31A-TPGK1 in pUSTE18p-Gγcyto-HIS3t |
45 |
| pUSTE18p-Gγcyto-Z955-H |
URA3-PSTE18-Gγcyto-Z955-TPGK1 in pUSTE18p-Gγcyto-HIS3t |
45 |
