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. 2016 Oct 26;6:36138. doi: 10.1038/srep36138

Figure 3. The effect of LCN2 overexpression in RBE cell proliferation and metastasis.

Figure 3

(A) LCN2 mRNA expression in RBE-CMV2 and RBE-LCN2 cells. Each value was a mean ± SD of three independent determinations. Experiment was done at least three times (**P < 0.01). (B) ELISA assay to determine the secreted LCN2 amounts of RBE-CMV2 and RBE-LCN2 cells. Each value was a mean ± SD of three independent determinations. Experiment was done at least three times (**P < 0.01). (C) The doubling time of RBE-CMV2 and RBE-LCN2 cells. (D) The migration and invasion ability of RBE-LCN2 cells as compared to RBE-CMV2 cells. Each value was a mean ± SD of three independent determinations. Experiment was done at least three times. Data was presented as the ratio to the control (RBE-CMV2 group) (**P < 0.01).