Figure 6. CCA patients had higher bile LCN2 level than patients with gall stones and LCN2 expression was negatively correlated with survival of CCA patients.

(A) The bile LCN2 level for CCA patients was statically higher than that for patients with gall stones (P < 0.001). (B) The AUC was 0.81 as analyzing bile LCN2 level by ROC curve. The optimal cutoff value of LCN2 that had a largest Youden index was 20.08 ng/ml, which had a sensitivity of 0.87 and false positive rate of 0.25 to differentiate CCA patients to non-CCA patients. (C,D) Immunohistochemical staining of human CCA specimen with different intensity scores for LCN2 expression (high and low, respectively; X400, Scale bar = 50 μm). (E) Kaplan–Meier plot of overall survival in CCA patients based on LCN2 expression levels. The high LCN2 expression group had poorer overall survival (P < 0.001).