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. 2016 Oct 6;17(4):1071–1086. doi: 10.1016/j.celrep.2016.09.058

Figure 3.

Figure 3

Upregulation of the E3 Ubiquitin Ligase FBXO32 and Its Transcription Factor FOXO3A in Endophilin Mutant Mice

(A) Transcript levels of Fbxo32 measured by qRT-PCR for indicated genotypes. ∗p < 0.05; Student’s t test; mean ± SEM.

(B) Upregulation of FBXO32 and FOXO3A in endophilin TKO whole-brain extracts. (Right) Quantification is shown (min. eight samples/genotype). ∗p < 0.05; ∗∗∗p < 0.005; Student’s t test; mean ± SEM.

(C) FOXO3A-[S253]P immunofluorescence reveals its altered distribution (shown by arrows) in brain sections of endophilin TKO hippocampus. Four mice/genotype are shown. The scale bar represents 10 μm.

(D) Silencing TKO neurons with Foxo3a siRNA (left: efficiency of knockdown by western blotting) decreases transcript levels of Fbxo32 measured by qRT-PCR. n = 3. ∗∗p < 0.01; Student’s t test; mean ± SD.

(E) Upregulation of FBXO32 in endophilin 1,2 DKO brains, at p0 (whole brain) and p14–p21 mice (cortex). (Right) Quantification is shown (eight samples/genotype). ∗p < 0.05; ∗∗p < 0.01; Student’s t test; mean ± SEM.

(F) Upregulation of FBXO32 in endophilin 1KO-2HT-3KO cortical extracts in 18-month mice. (Right) Quantification is shown (three samples/genotype). ∗p < 0.05; Student’s t test; mean ± SD.

See also Figures S2 and S3.