HRP treatment increased protein levels of a marker for adipogenesis in SCF of obese mice. (A) PPAR-γ1 and (B) PPAR-γ2 protein levels in adipose tissue. Equal loading of 30 µg of proteins was done in each well for Western blot. Data are normalized to tubulin protein levels and are presented as mean ± SE with n = 4–7 per group for PPAR-γ1 and PPAR-γ2 protein levels. *P < 0.05 compared with N diet; †P < 0.05 compared with saline. HF/HC, high-fat/high-carbohydrate diet; HRP, handle region peptide; N, normal; PGF, perigonadal fat; PPAR-γ, peroxisome proliferator-activated receptor-gamma; PRF, perirenal fat; SCF, abdominal subcutaneous fat.