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. 2016 May 8;9(8):e34091. doi: 10.5812/jjm.34091

Figure 1. Amplification of hES Coding Sequence and Construction of Expression Vector.

Figure 1.

A, Amplification of hES coding sequence using Pfu DNA polymerase. Lane 1: gene ruler DNA ladder mix (thermo scientific); lane 2: negative control; lane 3: amplified ORF of hES (592 bp). B, Construction of the pET26b-hES vector.