Table 3. BBD With Measured and Predicted Response of hES mRNA Expression Level^a.

Run	X1	X2	X3	Actual, %b	Predicted, %
1	0.2	4	1.1	71.52	92.09
2	1	4	1.1	204.32	200.04
3	0.2	10	1.1	207.52	211.80
4	1	10	1.1	208.80	188.23
5	0.2	7	0.8	272.96	261.13
6	1	7	0.8	295.84	308.86
7	0.2	7	1.4	92.64	79.62
8	1	7	1.4	104.48	116.27
9	0.6	4	0.8	289.92	281.19
10	0.6	10	0.8	306.24	313.78
11	0.6	4	1.4	80.32	72.77
12	0.6	10	1.4	139.36	148.1
13	0.6	7	1.1	376.16	332.64
14	0.6	7	1.1	312.80	332.64
15	0.6	7	1.1	308.96	332.64

^aX₁, IPTG concentration; X₂, post-induction time; X₃, cell density before induction (OD₆₀₀).

^bMean of duplicate observations.