Table 4. ANOVA for Response Surface Quadratic Model for hES mRNA Expression Levela.
| Source | D.F. | S.S. | M.S. | F-Value | Coef | SE Coef | T-Value | P-Value |
|---|---|---|---|---|---|---|---|---|
| Model | 9 | 270569 | 30063 | 65.07 | - | - | - | < 0.001 |
| Constant | - | - | - | 332.64 | 8.77 | 37.91 | < 0.001 | |
| X 1 | 1 | 7120 | 7120 | 15.41 | 21.09 | 5.37 | 3.93 | 0.001 |
| X 2 | 1 | 11646 | 11646 | 25.21 | 26.98 | 5.37 | 5.02 | < 0.001 |
| X 3 | 1 | 139947 | 139947 | 302.92 | -93.52 | 5.37 | -17.4 | < 0.001 |
| X 1 X 1 | 1 | 54672 | 54672 | 118.34 | -86.04 | 7.91 | -10.88 | < 0.001 |
| X 2 X 2 | 1 | 39955 | 39955 | 86.48 | -73.56 | 7.91 | -9.30 | < 0.001 |
| X 3 X 3 | 1 | 22441 | 22441 | 48.58 | -55.13 | 7.91 | -6.97 | < 0.001 |
| X 1 X 2 | 1 | 8649 | 8649 | 18.72 | -32.88 | 7.6 | -4.33 | < 0.001 |
| X 1 X 3 | 1 | 61 | 61 | 0.13 | -2.77 | 7.6 | -0.36 | 0.719 |
| X 2 X 3 | 1 | 913 | 913 | 1.98 | 10.68 | 7.6 | 1.41 | 0.175 |
| Lack of fit | 3 | 3536 | 1179 | 3.51 | - | - | - | 0.038 |
| Pure error | 17 | 5704 | 336 | - | - | - | - | - |
| Total | 29 | 279809 | - | - | - | - | - | - |
aX1, IPTG concentration; X2, post-induction time; X3, cell density in OD600; D.F., degrees of freedom; S.S, sum of squares; M.S., mean of squares; Coef., coefficient; SE.Coef., standard error coefficient; F-value, Fisher’s test; P-value, probability distribution value. The correlation coefficient (R2) was 0.967 and the adjusted R2 was 0.9521.