Figure 1. Differentially methylated regions in LAC.

The methylation level of 18 DMRs was investigated in 52 LAC primary tumors and 32 tumor-adjacent normal lung samples using MS-HRM analysis. The results of the methylation assessment are shown as stacked bar percentage plots for each DMR in (a–r). The relative proportion of samples in each category with 0–1% methylated templates are shown in white, 1–10% methylated templates in white with light grey stripes, 10–50% methylated templates in dark grey and 50–100% methylated templates in black. The statistical significance of the detected differences in methylation between groups was assessed using a Mann-Whitney test of ranks and two-tailed p-values ≤ 0.05 were considered statistically significant.