Table 1. DMRs selected for validation by MS-HRM analysis.
| ID | DMR |
MS-HRM assay location relative to known genes and CpG Islands (CGI) |
||||||
|---|---|---|---|---|---|---|---|---|
| No. | Diff. Methylation Score | Diff. Methylation Hypo (−)/Hyper (+) | Upstream | Intragenic | Downstream | CGI | Location (Hg38) | |
| OTX2 | 1 | 9.130 | + | OTX2 | Island | chr14: 56809871–56809962 | ||
| HOXD3 | 2 | 8.830 | + | HOXD3 | Shore | chr2: 176162676–176162747 | ||
| HOXB3/HOXB4 | 3 | 7.625 | + | HOXB3 | HOXB4 | Shore | chr17: 48577876–48577988 | |
| HOXD10 | 4 | 7.318 | + | HOXD10 | Island | chr2:176117574–176117651 | ||
| Chr1(q21.1).A | 5 | 6.765 | + | Island | chr1:147080162–147080262 | |||
| HIST1H3G/HIST1H2BI | 6 | 6.749 | + | HIST1H3G, HIST1H2BI | Island | chr6:26272252–26272379 | ||
| GHSR | 7 | 6.585 | + | GHSR | Island | chr3:172448360–172448447 | ||
| SIM1 | 8 | 6.191 | + | SIM1 | Island | chr6:100465031–100465125 | ||
| OSR1 | 9 | 6.117 | + | OSR1 | Island | chr2:19357150–19357251 | ||
| FRG1BP | 10 | 5.838 | − | FRG1BP | Island | chr20: 30377475–30377573 | ||
| Chr6(p22.1) | 13 | 5.653 | + | Island | chr6:28207550–28207669 | |||
| HOXA3 | 15 | 5.435 | + | HOXA3 | HOXA4 | Island | chr7: 27124232–27124343 | |
| LY75-CD302 | 17 | 5.223 | + | LY75-CD302, CD302 | LY75 | Island | chr2: 159797756–159797835 | |
| CTAGE15 | 18 | 5.217 | − | CTAGE15 | chr7: 143571685–143571797 | |||
| LOC648987 | 19 | 4.790 | + | ANXA2R | LOC648987 | Island | chr5: 43040396–43040521 | |
| HIST1H2AJ/HIST1H2BM | 21 | 4.635 | + | HIST1H2BM | HIST1H2AJ | Island | chr6:27814577–27814732 | |
| HOXA5 | 22 | 4.522 | + | HOXA5 | HOXA6 | Island | chr7: 27143474–27143563 | |
| HIST1H3E | 61 | 2.961 | + | HIST1H3E | Island | chr6:26225275–26225421 | ||