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(A) Protocol of our detection method to mouse and rat blood samples. (B) Detection using rat blood serum treated with acrolein over 0 (normal), 1, 20, or 60 days and compared with the ELISA method. Mean values with standard deviations are indicated. **p < 0.01; p values were determined by using two-tailed Student t test. (C) Detection of 4p by ESI-MS after reduction by 60 days’ sample. HRESI-MS m/z calcd for C8H5N3Ca [M+Ca]2+ 91.5049, found 91.5048.
