Fig. 5.

iSINE-mediated gene repression is independent of ADARs and STAUFEN1. a To test whether human iSINEs lead to gene repression in mouse cells, Nicn1 constructs were transfected in wild-type MEFs and luciferase activity was detected after 24 h. b mRNA levels of SINE-containing reporter genes were detected using RT-qPCR. c, d Nicn1-containing constructs were transfected in Adar1 ^−/−/Adar2 ^−/− mouse cells and after 24 h c protein and d RNA levels were measured. e, f Stau1 ^−/− MEF cells were also transfected and the e protein expression and f RNA levels were measured. Standard deviation is indicated. Asterisks indicate p values calculated with Student’s t-test: *p < 0.05, **p < 0.005, ***p < 0.0005