Fig. 1.

Changes in SKOv3 metabolism after induction of ARHI. SKOv3-ARHI cells pretreated with doxycycline or rapamycin were assayed using Seahorse XF24 analyzer to determine (a) ECAR and (b) OCR. The relative concentrations of intracellular metabolites at (c) 24 and (d) 48 h following ARHI induction were determined by ¹H-NMR, normalized to viable cell count, and expressed as a fold-change relative to non-induced cells (dotted red line). e The concentration of extracellular glutamate in SKOv3-ARHI media at 24 and 48 h post-induction was determined by ¹H-NMR, normalized to viable cell count, and expressed as a fold-change relative to non-induced SKOv3-ARHI media (dotted red line). f The concentration of extracellular ammonia (NH₃ + NH₄ ⁺) in SKOv3-ARHI media at 24 and 48 h post-induction was determined by fluorimetric assay; the ammonia concentration in non-induced media is shown by a dotted red line. The mean values for three replicates (n = 3) are shown along with the standard deviation. Statistical significance was determined by unpaired two-tailed t test in GraphPad (#, 0.1 > p > 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001)