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. 2016 Oct 26;16:824. doi: 10.1186/s12885-016-2850-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — ARHI induction alters glucose and choline metabolism. a SKOv3-ARHI cells were induced with Dox or uninduced for 48 h followed by the addition of ¹³C₆-glucose in the media. The intracellular water-soluble metabolite concentrations were determined by ¹H and ¹³C-filtered ¹H NMR spectra (1D HSQC) after 8 h, normalized to cell count, and expressed as a ratio between induced and uninduced samples. b Glycerophosphocholine:phosphocholine (GPC/PC) ratio in water-soluble extracts of SKOv3-ARHI cells transfected with shAtg5 or a control shRNA. Data was obtained by ¹H NMR of water soluble metabolite extracts of SKOv3-ARHI between 24 and 48 h in culture. Three replicate samples were used for each condition to calculate the mean and standard deviation and statistical significance was determined by two-way ANOVA with p-values adjusted for multiple comparisons (*, p < 0.05; **, p < 0.01)