Table 3.

Non-synonymous (d_N) and synonymous (d_S) substitutions (± standard deviation) for C. perspicillata, D. rotundus and M. molossus

Species	Region	N	dN ± SD	dS ± SD	dN/dS	Z-test	p-value
C. perspicillata
	ABS	25	0.405 ± 0.131	0.082 ± 0.064	4.939	5.119	<10-5
	Non-ABS	64	0.121 ± 0.047	0.043 ± 0.029	2.792	3.283	0.0013
	All	89	0.187 ± 0.053	0.0528 ± 0.026	3.548	5.619	<10-5
D. rotundus
	ABS	25	0.314 ± 0.116	0.077 ± 0.055	4.081	3.811	0.0002
	Non-ABS	64	0.049 ± 0.035	0.045 ± 0.032	1.075	0.156	0.8764
	All	89	0.114 ± 0.470	0.054 ± 0.032	2.119	2.713	0.0077
M. molossus
	ABS	21	0.323 ± 0.103	0.068 ± 0.058	4.753	4.080	0.0001
	Non-ABS	60	0.060 ± 0.021	0.056 ± 0.031	1.078	0.186	0.8528
	All	81	0.120 ± 0.028	0.058 ± 0.030	2.049	2.599	0.0105

Putative ABS and non-ABS were identified assuming functional homology to the human ABS of the HLA-DR1 molecule Brown et al. [65]. N is the number of codons in each sequence region. Z-test values and p-values are shown