Table 4.

Oligonucleotide primers

Name	Sequence (5′–3')a	Restriction site
araL-F	gggaattccatatgcgtattatggccagtcatgat	NdeI
araL-R	ccgctcgagtatcagaatcccctcctcagc	XhoI
yutF-F	cgcggatccatgaaaacatataaagggtattta	BamHI
yutF-R	cccaagcttaatgtatggaatccattcagt	HindIII
ysaA-F	cgcggatccatgaaagccgtattttttgattta	BamHI
ysaA-R	ccgctcgagtttctctaatataggaaacaa	XhoI
YqeG-F	gggaatccatatgttaaaaaagtttttt	NdeI
YqeG-R	cccaagcttttactcctcccactgaat	HindIII
ycsE-F	gggaattccatatgtctgtccaaagagaagatgta	NdeI
ycsE-R	ccgctcgagtagtacccaatggcgaatcgcttta	XhoI
yktC-F	cgcggatccatgacaaattggacggaaatcgat	BamHI
yktC-R	cccaagcttcttccgagcatgaagatactc	HindIII
ycsE-1	gtttattgatggcgtgac
ycsE-2	ctgtgtagccttgagagtgatgaacagacatatatgtacctctc
ycsE-3	tcactctcaaggctacacag
ycsE-4	cgcaagcttcaaaaattatatggag
ycsE-5	ctccatataatttttgaagcttgcggggtactataaaaaaagagagtcc
ycsE-6	aacacttcatttgcggtc
yktC-1	gttcgcctagagctgtaagcttc
yktC −2	gccgatgataagctgtcaaagcaatctcatcgatttccgtcc
yktC −3	tttgacagcttatcatcggc
yktC −4	ttataaaagccagtcattaggc
yktC −5	gcctaatgactggcttttataagtgctagccggaaacccatc
yktC −6	gaacatctatcaccagacgacatc
yqeG-d1	gacataatgcgccatttgatggttg
yqeG-d2	gcttgagtcaattccgctgtcgctcaaacgaaaagggcacattcag
yqeG-d3	cgacagcggaattgactcaagc
yqeG-d4	cgcaagcttacgataaacccagc
yqeG-d5	gctgggtttatcgtaagcttgcgcaaaacccgcacccttccc
yqeG-d6	caggcagagcatatggatacg
yqeG-s1	ccttccagggtatgtttctc
yqeG-s2	gatactgcactatcaacacactctttcgacatggatgagcgatgatg
yqeG-s3	aagagtgtgttgatagtgcagtatc
yqeG-s4	caacaagctggggatccg
yqeG-s5	cggatccccagcttgttgcgacaatcattttgaaagaaagaaaaaggg
yqeG-s6	gatgacctcgtttccaccggcaaccttttccatttcttactcctcc
yqeG-s7	ccggtggaaacgaggtcatc
yqeG-s8	cacaaattaaaaactggtctgatcggcctaactcacattaattgcgttg
yqeG-s9	cgatcagaccagtttttaatttgtg
yqeG-s10	ttaacaaaattctccagtcttcacatcg

^aRestriction enzyme cleavage sites are underlined